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KMID : 0364820230590030214
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Korean Journal of Microbiology
2023 Volume.59 No. 3 p.214 ~ p.216
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Complete genome sequence of Bacillus velezensis 2F-NA isolation from the soil of a livestock farmhouse
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Lee Gyu-Dae
Park Tae-Hyung
Kim Min-Ji
Shin Jae-Ho
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Abstract
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Bacillus velezensis 2F-NA was isolated from the soil of a livestock farmhouse in Republic of Korea. Long read sequencing was conducted using the Nanopore MinION device, resulting in the generation of a complete genome with a size of 3,933,901 bp through de novo assembly. The genome exhibits a G + C content of 46.5% and contains 3,004 protein-coding, 30 rRNA, 91 tRNA, and 5 ncRNA genes. Moreover, the NCBI Prokaryotic Genome Annotation was used to predict the presence of genes involved in plant growth promotion, such as urease subunit encoding, Indole-3-acetic acid (IAA) production pathway , and nitrate reductase related genes.
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KEYWORD
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Bacillus velezensis 2F-NA, complete genome, nanopore, whole genome sequencing
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